Kiholo and Puako Field Work

November 19 and 20

Three members of the NOAA Marine Turtle Biology and Assessment team came over to join our HPA team for two days of field work to capture and tag honu at Kiholo Bay and 22 Puako Beach Road, Puako, Hawaii.

On Tuesday (11/10/19) We went to Kiholo bay hosted by TNC's Biologist, Barbara Siedel.  We arrived around 0900 hours, set up our work area and began capture and sampling work around 0930 h.
During the time from 0930 to around 1400 hours, we were able to capture and sample 10 honu.  The honu ranged in size from 5.4 kg to 40.8 kg and, of the 10 honu captured 5 were recaptures and 5 were new captures.  We were able to obtain blood and tissue samples from all 10 animals and all of them were tagged with oxytetracycline (a bone growth marker).  All animals were safely and harmlessly released with appropriate mototool numbers 82 to 89.
During the late morning and early afternoon, the weather turned a little sour and there was significant thunder and lightening which caused us to reduce and finally cancel our efforts at capturing and working up honu. 


On Wednesday, 11/20/19, we picked up a second team of HPA students from the upper and middle schools and joined the MTBAP personnel once again.  This time we traveled to 22 Puako Beach Road and set up a working area at the public access area.  The weather was clear and there was a strong onshore wind with moderate swells from the west.  The tide was quite high which we thought might be a problem but we now think it was a blessing because the honu were in the area feeding in the morning.
The total for the two days was 21 honu captured, sampled and harmlessly released.    

We started capture work around 0930 h and were lucky to get animals on a pretty regular basis right up to 1200 h when we decided to break for lunch.  
At the end of the day, we had been able to capture and sample 11 honu.  10 of the 11 were new with only 1 recapture.  The size range was narrower that Tuesdays sample at Kiholo with a size range of 9.6 to 34.2 kg.  The size of the animals was more towards the large juvenile range with only one really small critter capture.
We were able to obtain samples from all of the animals and the mototools were applied (89 to 100!!!!)


Images from the two days.

The video above is a short sequence showing Ms. Jim, students and a NOAA scientist snorkel capturing a juvenile honu for weighing, measuring and tagging.  

Ms Jim loads an external tag applicator prior to applying a metal tag to the left hind flipper.

Ivanni explains what we are doing to some visitors.

The Tagging Team at Puako.

Recent Tagging Trips-

During the week of October 22 to 24, HPA's STRP program joined the NOAA Marine Turtle Biology and Assessment Program to conduct three days of capture and sampling at Hualalai, Waikoloa and Mauna Lani.
The primary purpose of the field work was to collect blood samples to continue the sex ration study started back in April.  During the trips, HPA students assisted Laura Jim, Marc Rice (HPA) and Shawn Murakawa, Ali Balis and Camryn Allen (NOAA) to capture, tag, measure and draw blood samples from green turtles.
On Tuesday we went to Hualalai where we were hosted by the wonderful Four Seasons Resort team headed by David Chai.  During the day we were able to capture 7 honu.  All turtles were worked up (measured, weighed and blood drawn) and the HPA students Hikari Shaver, Ivanni Jamin and Jimmy Liu did the lab work that afternoon and froze the plasma collected.
On Wednesday we went to the Waikoloa Hilton where we were hosted by Tessa Cazimero (Hilton Biologist).  We were able to capture and work up 12 honu noting that they were well feed and quite plump!  The blood samples were worked up that afternoon by the same exemplary HPA team.
On Thursday, our last day of field work, we went to Mauna Lani Bay and were able to capture 8 honu.  After completing all of the field measurements and drawing blood, we returned to the lab and worked up the blood samples.
In all, we were able to collect 27 samples and worked up that number of turtles.
The team effort (HPA and NOAA) worked very well and everyone learned a lot from our visiting NOAA scientist.

Below are a few pictures from the three days of field work.

Hikari pipettes blood serum after centrifuging.  The serum is
put into cryovials and frozen in liquid nitrogen.

Charlotte returns a honu to the ocean after it was weighed and measured and a blood sample was taken.

The team of HPA students controls a small turtle on the work-up table.

Our worksite at Hualalai.

Our data taker!

Students control a honu while a harmless identifying number is etched into its shell.

The team releases a honu.

Ms Jim etches a number into the carapace.

Lunch time at the Waikoloa Hilton Hotel.  We all enjoyed Laura's fresh squeezed juice!!!

Roughing it during lunch at the Waikoloa Hilton.

Our work area at the Waikoloa Hilton during High tide.

A student working on a honu at the Mauna Lani Bay site.

River takes a small tissue sample from the shoulder of a honu.

Ivanni accurately taking down data.

Mariko assists Ms Jim in taking data.

Jimmy does an oral exam.

Mr. Balazs took these pictures of the turtles feeding on algae at 0730 in the morning at the Waikoloa Hilton Water Fall.

Kiholo Bay Tagging Trip

Kiholo Bay, September 26, 2019

A team of six HPA students (4 upper school and 2 middle school), Laura Jim and Marc Rice traveled to Kiholo Bay and conducted a sea turtle capture and tagging event.  This is part of the ongoing area monitoring program and the Hawaiian Honu Sex Ratio Project.  As mentioned in earlier blogs, since turtles sex is determined by the temperature of incubation, there is fear that increasing environmental temperatures might cause more female turtles to be produced and cause the feminization of the population.  To determine if this is true, we are joining NOAA's Marine Turtle Biology and Assessment Program to try and determine the current sex ratio of juvenile and sub-adult turtles in Hawaii.
This trip, in addition to monitoring honu health and growth, was focused on obtaining blood samples from each captured turtle in order to determine its sex.
During the trip, we captured and worked-up 8 turtles.  5 of them were recaptures and 3 were new captures. Blood samples were obtained from 6 of the honu.
A special thanks to the Nature Conservancy for hosting our team during the day and for always being so cooperative and welcoming.

Below are a series of images of our days work.

Kiholo Bay above with the arrow showing the location of our sampling area.

Ms. Jim retrieves a honu from the net while students assist with a carrying container.

It is protocol that blood samples should be drawn withing 15 minutes of capture to lessen the effect on hormone levels.  Here an HPA student draws 6 ml. of blood from the dorsal blood sinus.

After the blood is drawn, students weigh  the honu.

Students assisting with the assessment of a honu.

Using a bird speculum to look in the mouth.

Students help to control a honu while Marc Rice obtains a skin tissue sample for DNA analysis.

The head of a honu is gently supported by Penelope while the ventral measurements are taken.

A honu, all samples and measurements taken, is safely and harmlessly released back into the environment.

Releasing another honu.

Releasing

Releasing a Honu.

The HPA team along with Barbara Sidel (The Nature Conservancy) our host for the day.

Back in the laboratory at HPA, the blood samples are spun and the plasma extracted and frozen at -40 degrees C for later analysis of testosterone levels.

Pipetting off the supernatant plasma.

The team giving a shout out to one of our alum benefactors for his donation of the centrifuge, without which this project would not be possible.

Mauna Lani Keiki Honu Monthly Checkup

August 27, 2019

Once again we joined Pi'i Laeha at the Mauna Lani Bay Hotel to  assist in the monthly measurement of the 6 yearling Sea Life Park honu that are in residence at the hotel.  Laura Jim's Marine Turtle Enrichment class assisted in the weighing and measureing of the 6 little turtles.  Each group of students had a picture of the right and left side of the head head of one of the little honu and had to identify which turtle we were measuring based on the head scale pattern.
It was a beautiful day and everyone had a great time assisting in the monthly evaluation.
Below are a series of images of the activities of the day.